Application

Anti-Adiponectin Antibody detects level of Adiponectin & has been published & validated for use in ELISA & WB.

Research CategoryMetabolism

Research Sub CategoryLipid Metabolism & Weight Regulation

Western blot: 1:5,000-1:10,000 using ECL. Reacts with the ~35 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:5,000-1:10,000.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant mouse adiponectin from E. coli.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABS176

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin in PBS, pH 7.4.

Format: Purified

Specificity

Recognizes mouse adiponectin. Recognizes both natural and recombinant protein.

SPECIES REACTIVITIES:

There is weak reactivity with rat. Reactivity with other species has not been confirmed.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.